Comprehensive definitions of analytical methods, quality documentation, storage protocols, and regulatory terminology used in peptide research.
A chemical modification involving the addition of an acetyl group, typically at the N-terminus. Acetylation can protect against enzymatic degradation and improve peptide stability.
A measured portion of a larger sample, typically prepared to avoid repeated freeze-thaw cycles of the main stock. Aliquoting peptide solutions into single-use portions protects against degradation.
A chemical modification where the C-terminal carboxyl group is converted to an amide (-CONH2). Many bioactive peptides are amidated to improve stability and receptor binding affinity.
The building blocks of peptides and proteins. There are 20 standard amino acids, each with unique properties. The sequence and combination of amino acids determines peptide structure and function.
A unique identifier assigned to a specific production batch. Batch numbers enable complete traceability and are essential for quality investigations and research documentation.
The end of a peptide chain with a free carboxyl group (-COOH). Many peptides have modifications at the C-terminus, such as amidation.
A formal document issued by quality control that confirms a product batch meets specified quality standards. For peptides, COAs typically include HPLC purity, MS confirmation, appearance, and batch information.
A graphical representation of detector response over time during chromatographic separation. Peaks represent individual compounds, with peak area proportional to concentration. Peptide purity is calculated from the ratio of the main peak area to total peak areas.
A temperature-controlled supply chain that maintains products within specified temperature ranges from manufacturing to delivery. Critical for maintaining peptide integrity during shipping.
Adherence to applicable laws, regulations, guidelines, and specifications. For research peptides, compliance involves proper labeling, documentation, and restriction to research applications only.
A unit of molecular mass equal to one-twelfth the mass of a carbon-12 atom. Peptide molecular weights are typically expressed in Daltons or kiloDaltons (kDa).
Chemical breakdown of a peptide through reactions such as hydrolysis, oxidation, deamidation, or aggregation. Proper storage conditions minimize degradation and maintain compound integrity.
A hygroscopic substance used to absorb moisture and maintain dry conditions. Desiccants protect lyophilized peptides from moisture uptake during storage.
A covalent bond formed between the sulfur atoms of two cysteine residues. Disulfide bonds help stabilize peptide structure and are critical for proper folding of many biologically active peptides.
An ionization technique used in mass spectrometry where the analyte solution is sprayed through a charged needle, creating charged droplets that evaporate to produce gas-phase ions. Commonly used for peptide and protein analysis.
A system of quality assurance ensuring products are consistently produced and controlled according to quality standards. GMP-grade peptides follow documented procedures for manufacturing and testing.
The federal department responsible for helping Canadians maintain and improve their health, including regulation of therapeutic products. Research peptides are not regulated as therapeutic products when sold for research purposes only.
An analytical technique used to separate, identify, and quantify components in a mixture. In peptide analysis, HPLC is the primary method for determining purity by measuring the relative abundance of the target peptide versus impurities. Results are typically expressed as a percentage.
Chemical breakdown through reaction with water, which can cleave peptide bonds and reduce purity. Lyophilization and low-moisture storage protect against hydrolytic degradation.
Any substance present in a peptide sample other than the intended product. Sources include synthesis byproducts, degradation products, truncated sequences, and process-related contaminants.
Latin for 'in glass,' referring to studies performed outside of living organisms, typically in test tubes, cell cultures, or other controlled laboratory environments. Research peptides are intended for in vitro use only.
A test used to detect bacterial endotoxins using lysate from horseshoe crab blood cells. Important for peptides intended for cell culture or injection studies to ensure absence of pyrogenic contamination.
A combined analytical technique that couples liquid chromatography separation with tandem mass spectrometry detection. Provides both quantitative purity data and structural confirmation in a single analysis.
A dehydration process that removes water from a frozen peptide solution through sublimation. Lyophilized peptides are more stable for storage and shipping than solutions, with extended shelf life.
An ionization technique where the sample is mixed with a matrix material and irradiated with a laser, causing desorption and ionization. Often used for larger peptides and proteins.
An analytical technique that measures the mass-to-charge ratio of ions to determine molecular weight and structure. For peptides, MS confirms molecular identity by comparing observed mass to theoretical mass calculated from the amino acid sequence.
The sum of atomic weights of all atoms in a molecule, expressed in Daltons (Da). Peptide molecular weight is calculated from the amino acid sequence and confirmed by mass spectrometry.
A document containing information about potential hazards, safe handling procedures, storage requirements, and emergency measures for chemical substances. Required for regulatory compliance in Canadian laboratories.
The end of a peptide chain with a free amino group (-NH2). Peptide sequences are conventionally written from N-terminus to C-terminus.
A chemical reaction involving the loss of electrons, often resulting in damage to methionine and cysteine residues. Oxidation can be minimized by storing peptides under inert gas or with antioxidants.
A covalent bond formed between the carboxyl group of one amino acid and the amino group of another through a dehydration reaction. This bond links amino acids together to form peptide chains.
The linear sequence of amino acids in a peptide or protein, read from the N-terminus to the C-terminus. This sequence determines all higher-order structural properties.
The proportion of target compound relative to total material, typically expressed as a percentage. Research-grade peptides generally have purity ≥98% as determined by HPLC analysis.
The process of dissolving lyophilized peptide powder in an appropriate solvent to create a solution for research use. Common solvents include sterile water, bacteriostatic water, or specific buffers depending on peptide properties.
A known or expected impurity, typically arising from the synthesis process. Related substances may include deletion sequences, incomplete deprotection products, or modification variants.
A designation indicating that a product is intended exclusively for laboratory research and is not approved for human consumption, therapeutic use, or diagnostic purposes. All research peptides carry this classification.
A single amino acid unit within a peptide chain. The term 'residue' refers to what remains of an amino acid after losing a water molecule during peptide bond formation.
The time required for a compound to travel through a chromatographic column and reach the detector. Each compound has a characteristic retention time under specific conditions, aiding in identification.
The ability of a peptide to maintain its specified properties over time under defined storage conditions. Stability is affected by temperature, light exposure, moisture, and chemical environment.
Independent laboratory analysis performed by a facility separate from the manufacturer. Third-party testing provides unbiased verification of quality specifications and supports research credibility.
Canada's national hazard communication standard for hazardous workplace chemicals. WHMIS requires proper labeling, MSDS availability, and worker training for handling chemical substances.
