TB-500

TB-500 peptide Canada: a synthetic version of a 43-amino acid region of Thymosin Beta-4 (TB4), a naturally occurring protein involved in cell migration and differentiation research. Available from Maple Research Labs. Published third-party analytical reports for tested batches are indexed on our Certificates of Analysis page.

Product Specifications

• Sequence Region: Thymosin Beta-4 active fragment
• Molecular Weight: Approximately 4963 Da
• Analytical documentation: Linked report tested a 10mg sample; it does not establish 5mg coverage.
• Form: Lyophilized powder
• Quantity: 5mg per vial

TB-500 Peptide Canada Research Summary

TB-500 is a synthetic peptide representing the active region of Thymosin Beta-4, a 43-amino acid protein that is expressed in virtually all mammalian cell types. Thymosin Beta-4 was first isolated from calf thymus tissue and has since been identified as one of the most abundant intracellular peptides in mammalian systems. The TB-500 peptide Canada product contains the key actin-binding domain responsible for many of the observed biological activities in research settings.

Research has focused on TB-500’s interaction with globular actin (G-actin), which plays a central role in cytoskeletal dynamics. Published studies have demonstrated that Thymosin Beta-4 promotes actin polymerization and cell motility in multiple cell types, including endothelial cells, keratinocytes, and fibroblasts.

Mechanism of Action Research

The primary molecular mechanism under investigation involves TB-500’s ability to sequester G-actin monomers, thereby regulating the actin cytoskeleton dynamics essential for cell migration and morphogenesis. The key active sequence (LKKTETQ) within TB-500 has been identified as the domain responsible for actin binding activity observed in in vitro assays.

Published preclinical studies have examined TB-500 in cell-migration, actin-binding and vascular development assays. In vitro studies have demonstrated effects on endothelial cell migration and differentiation in tube formation assays.

Additional research has investigated the relationship between Thymosin Beta-4 and various signaling pathways, including the Akt/PI3K pathway and modulation of inflammatory mediators. Studies in cardiac tissue models have examined the peptide’s interactions with epicardial progenitor cell populations.

Preclinical Study Summary

TB-500 and its parent protein Thymosin Beta-4 have been the subject of extensive preclinical investigation. The peptide’s well-characterized interaction with the actin cytoskeleton has made it a research tool for studying cell migration and cytoskeletal dynamics.

All published findings referenced here are based on animal models and in vitro systems. Researchers should consult primary literature sources for experimental protocols and complete findings.

References

• Goldstein AL, et al. Thymosin beta4: a multi-functional regenerative peptide. Expert Opin Biol Ther. 2012 (PubMed)

TB-500 Peptide Canada Quality Assurance

Third-party certificates of analysis for tested lots are published on our COA page. Check the report’s compound, tested size, date and method. Vials do not carry printed lot or batch numbers. Linked report tested a 10mg sample; it does not establish 5mg coverage. HPLC-UV area-percent is a method-specific relative-area result; it does not independently establish molecular identity or sterility, endotoxin status, or safety.

Storage and Handling

Store lyophilized TB-500 peptide at -20C for long-term storage. Avoid repeated freeze-thaw cycles. Keep away from direct light and moisture.

Shipping

Orders placed by 2 PM PT (Mon–Fri) ship the same business day, from BC with tracking. All orders include temperature-controlled packaging.

Certificate of Analysis (COA)

Linked report tested a 10mg sample; it does not establish 5mg coverage. View the tested 10mg sample report. A matching public 5mg sample report has not been established in this review.

For research purposes only. Not for human consumption. Not for diagnostic or therapeutic use.

Related Research Articles

• BPC-157 vs TB-500: Research Comparison
• TB-500 vs BPC-157/TB-500 Blend: Research Comparison
• Understanding Peptide Purity: Why It Matters for Research
• Certificates of Analysis (COA) Documentation
• Browse All Research Peptides

Frequently Asked Questions

What is the CAS number for TB-500?

The CAS registry number for TB-500 (Thymosin Beta-4 fragment) is 77591-33-4. TB-500 is the synthetic version of the naturally occurring 43-amino acid peptide Thymosin Beta-4.

How is TB-500 purity tested?

Published 10mg sample report: DBAV-TB500-10-051226 (tested May 23, 2026), HPLC-UV relative-area result 98.62%; expected content 10.00mg, measured content 9.84mg. Linked report tested a 10mg sample; it does not establish 5mg coverage. These sample results do not establish current shipping-unit or stock-lot coverage.

How should TB-500 be stored?

Store lyophilized TB-500 at -20C for long-term storage (18+ months stability). TB-500 is a larger peptide and slightly less stable in solution than smaller compounds.

What is TB-500 studied for in research?

TB-500 has been studied for its role in cell migration and blood vessel formation in cell and animal models. Research has focused on its interaction with actin, a key protein in cell structure and motility.

Related Research Peptides from Maple Research

Researchers may also be interested in TB-500 10MG, BPC-157 + TB-500 Blend, and BPC-157 10MG. Third-party certificates of analysis for tested lots are published on our COA page. Orders placed by 2 PM PT (Mon–Fri) ship the same business day, from BC with tracking.